[B-CG03] Earth and Planetary Science Frontiers for Life and Global Environment

[BCG03-P03] Recent development of seamless procedures on wet chemistry, cation-exchange chromatography and ion-pair chromatography with high-precision mass spectrometry for short-chain peptide molecules.

*Yoshinori Takano¹ (1.Japan Agency for Marine-Earth Science and Technology (JAMSTEC))

Keywords:short-chain peptide, LC/ESI-MS analysis , target and non-target analysis

We report seamless analytical procedures for characterization of underivatized short-chain peptides by wet chemical treatment and ion-pair liquid chromatography with electrospray ionization–mass spectrometry. The chromatographic separation via a porous graphitic carbon stationary column eluted using an acetonitrile solvent gradient revealed a positive correlation between the retention time and the degree of hydrophobicity reflected in a distinct relationship between methylene elongation of amino acids. We also validated the chromatographic separation utilizing other stationary column and high-resolution mass spectrometry, which supports the analytical accuracy for identification. By eliminating the inorganic and organic matrix effect via cation-exchange chromatography, we can avoid ion suppression and enhancementto demonstrate accurate analytical evaluations with robust mass spectroscopic responses. The developed method provides new insight into short-chain peptide chemistry that can be utilized for numerous interdisciplinary research applications.

Please see the recent publication for more in detail [1]. The preliminary reports in this paper were presented at the 29th International Meeting on Organic Geochemistry at Gothenburg, Sweden in September 2019. This work was supported by the Japan Society for the Promotion of Science (JSPS). This is a joint work with Y. Oba, S. Furota, H. Naraoka, N. O. Ogawa, T. M. Blattmann, and N. Ohkouchi.

[References]

[1] Takano et al. (2021) Analytical development of seamless procedures on cation-exchange chromatography and ion-pair chromatography with high-precision mass spectrometry for short-chain peptides. International Journal of Mass Spectrometry, doi: 10.1016/j.ijms.2021.116529.