*Taiki Katayama1, Hideyoshi Yoshioka1, Masanori Kaneko1, Susumu Sakata1, Miki Amo2, Tetsuya Fujii2
(1.National Institute of Advanced Industrial Science and Technology, 2.Japan Oil, Gas and Metals National Corporation)
Keywords:gas hydrate, biogenic methane formation, cultivation, deep subseafloor sediment
Gas hydrates deposited in subseafloor sediments are considered to be the Earth’s largest natural gas reservoir of biogenic methane based on geochemical analyses. However, thus far, viable methanogens, microorganisms that are responsible for biogenic methane formation, have been poorly obtained and characterized. Characterization of living methanogens in subseafloor sediments at hydrate sites are fundamental for understanding biogenic gas hydrate formation and our estimations of gas hydrate reserves. We performed cultivation dependent and independent analyses of microorganisms in the hydrate-bearing sediments from the eastern Nankai Trough. In this presentation, I will show previously overlooked phylogenetic and metabolic diversity of viable methanogens and importance of methylotrophic methanogenesis and discuss their depth-related profiles. This study was part of the Research Consortium for Methane Hydrate Resources program in Japan (MH21 Research Consortium).