[H-CG29] Advanced life support systems with closed bio-ecosystems and thier applications

[HCG29-P02] Gene expression analysis of Nostoc sp. HK-01 in drought stress

*Kengo Shibasaki¹, Hiroko Tanaka¹, Hiroshi Katoh², Kaori Tomita-Yokotani³, Midori Ong³, Tomoko Abe¹ (1.School of Science and Engineering, Tokyo Denki University, 2.Organization for the Promotion of Regional Innovation, Mie University, 3.Faculty of Life and Environmental Sciences, University of Tsukuba)

Keywords:cyanobacteria, drought tolerance

A strain of the terrestrial cyanobacterium Nostoc sp. HK-01 could be used for bio-chemical circulation in a closed ecosystem including space. Furthermore, its high tolerance to water-deficit environments has ability of usefulness as a food. In this study, genes of Nostoc sp. HK-01 induced by drought stress were explored.
Nostoc sp. HK-01 cells were dehydrated slowly. Proteins or RNAs were extracted from dehydrated or wet HK-01 cells. Extracted proteins are applied to SDS-polyacrylamide gel electrophoresis. In comparison with the wet cell proteins, significant abundance changes of some proteins in the dehydrated cells were detected. In addition, many genes increased level of expression in dehydrated cells were detected by RNA-sequencing.

References:
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3. Kimura, S. Tomita-Yokotani, K. et al., Am. J. Plant Sci. 8, 2695-2711, 2017.
4. Tomita-Yokotani, K. et al., Astrobiology 21, 1506-1514, 2021.