[M-IS08] Geopark

[MIS08-07] Clarification of phylogenetic relationship and genetic diversity of daylily native to Japan associated with Geopark

*Tsuneo Sasanuma^1,2, Kana Tamagawa^1,2, Yuki Sato¹, Noa Saito¹, Seiji Kishimoto^3,4 (1.Facluty of Agriculture, Yamagata University, 2.Graduate School of Agriculture, Yamagata University, 3.Faculty of Life Design, Tohoku Institute of Technology, 4.Mt. Chokai and Tobishima Island Geopark Promotion Council)

Keywords:Daylily, Tobishimakanzo, Geopark network, DNA analysis

Daylily (Hemerocallis dumortieri) whose Japanese name is Zenteika is a mountainous plant native to the Central and Northeastern Japan. It is commonly known as its common name Nikkokisuge. On the other hand, in Tobishima and Sadogashima, isolated islands in Sea of Japan, there is growing Tobishimakanzo, which is an island variety of Zenteika,. In addition, Zenteika that grows naturally in Hokkaido is called Ezozenteika, and is morphologically distinct from that of Honshu. Tobishimakanzo, Zenteika and Ezozentei have become symbolic plants of their native regions, and are well known and have a high level of public interest. Contrary to their high level of popularity, there is little scientific knowledge regarding their genetic characteristics. The natural habitat of Tobishimakanzo, Zenteika and Ezozenteika is located in geoparks of Mt. Chokai and Tobishima, Sado, the foothills of Mt. Kurikoma, Oga Peninsula and Ogata, Shimokita Peninsula, and Mt. Apoi, and as rare plants, they are also the subject of geopark scientific study. In this study, we associated with Japan Geoparks Network and collaborate with geoparks around the country to investigate the phylogenetic relationships and genetic diversity of the group of Zenteika, which is a symbol plant of the region and is a suitable material for nature education, through DNA analysis and survey of morphological traits in their native habitat. DNA analysis was performed by sequencing two regions of the chloroplast intergenic region and fragment analysis at the whole genome level. Samples were collected from over 50 populations in 10 prefectures, and the phylogenetic relationships and genetic diversity of Zenteika in each region were investigated. As a result, we revealed that the Tobishimakanzo is a variety of Zenteika as previously having been thought, and that there is no clear genetic differentiation between Zenteika in Honshu and Ezozenteika indicating that Ezozenteika should be considered as the same taxonomic group of Zenteika. Based on polymorphism in chloroplast DNA, the major intraspecific differentiation of Zenteika has occurred not between Hokkaido and Honshu as previously thought, but in the southern part of Tohoku. Furthermore morphological differentiation, that is, flower branch length, has also occurred in the southern part of Tohoku as the border. The results of this study have been widely disseminated to citizens through lectures and other events conducted through geopark activities.