[EP-15] SECRETOME DERIVED FROM DENTAL PULP STEM CELLS AND ITS POTENTIAL PROMOTING INITIAL BONE HEALING AND ACCELERATE THE OSSEOINTEGRATION OF DENTAL IMPLANTS
[Abstract]
[Objective]
Numerous elements, including immunological components of materials, cytokines, and inflammatory agents generated by implants, have been thought to influence the host respons.1 secretome, a substance released from mesenchymal stem cells, contains extracellular vesicles, cytokines, and growth factors that support multiple tissue regeneration processes. 2, The aim of this study is to examine the potential of the secretome derived from dental pulp stem cells (DPSC) to promote early healing processes and quicken bone maturation in dental implants.
[Method]
Six male wild-type mice had their right condyle femurs implanted. Secretome is applied on gelatin sponge (Hemosponge®) and placed at the peri-implant locations. A RealTimePCRarray was conducted 7th days after surgery to evaluate the expression of cytokine and chemokine receptors. On day 28, implant osseointegration was qualitatively evaluated using HE staining. The t-test was used to assess the statistical significance.
[Results and Discussion] qPCR was used to evaluate the expression of these receptors on the 7th day after implant placement. The presence of an immune reaction phase in the implant area that was given a secretome was proven by a significant increase in the expression of cytokines and chemokine receptors. This response is initially necessary for cleaning the wound and initiating the healing process.4,5 Furthermore, on day 28, new bone formation or woven bone appeared around the collagen fibers in the control group. Meanwhile, in the group implanted with secretome, collagen fibers had been replaced by mature bone. The results of this study indicate that the secretome of DPSC can induce initial healing process and accelerate osseointegration of dental implant.
[References]1). Wei F, Liu S, Chen M et al. Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling. Front. bioeng biotechnol 2021; 9:1-19 2). Gwam C, Mohammed N, Ma X. Stem Cell Secretome, Regeneration, and Clinical Translation: A Narrative Review. Ann Transl Med 2021;9:70. 3). Loi F, Córdova LA, Pajarinen J, et al. Inflammation, fracture and bone repair. Bone. 2016;86:119–130.4). Ridiandries A, Tan JTM, Bursill CA. The Role of Chemokines in Wound Healing. Int J Mol Sci 2018;19:3217 5). Biguetti CC, Cavalla F, Silveira EM et al. Oral implant osseointegration model in C57Bl/6mice: microtomographic, histological, histomorphometric and molecular characterization. J Appl Oral Sci 2018;26:e20170601.
[Objective]
Numerous elements, including immunological components of materials, cytokines, and inflammatory agents generated by implants, have been thought to influence the host respons.1 secretome, a substance released from mesenchymal stem cells, contains extracellular vesicles, cytokines, and growth factors that support multiple tissue regeneration processes. 2, The aim of this study is to examine the potential of the secretome derived from dental pulp stem cells (DPSC) to promote early healing processes and quicken bone maturation in dental implants.
[Method]
Six male wild-type mice had their right condyle femurs implanted. Secretome is applied on gelatin sponge (Hemosponge®) and placed at the peri-implant locations. A RealTimePCRarray was conducted 7th days after surgery to evaluate the expression of cytokine and chemokine receptors. On day 28, implant osseointegration was qualitatively evaluated using HE staining. The t-test was used to assess the statistical significance.
[Results and Discussion] qPCR was used to evaluate the expression of these receptors on the 7th day after implant placement. The presence of an immune reaction phase in the implant area that was given a secretome was proven by a significant increase in the expression of cytokines and chemokine receptors. This response is initially necessary for cleaning the wound and initiating the healing process.4,5 Furthermore, on day 28, new bone formation or woven bone appeared around the collagen fibers in the control group. Meanwhile, in the group implanted with secretome, collagen fibers had been replaced by mature bone. The results of this study indicate that the secretome of DPSC can induce initial healing process and accelerate osseointegration of dental implant.
[References]1). Wei F, Liu S, Chen M et al. Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling. Front. bioeng biotechnol 2021; 9:1-19 2). Gwam C, Mohammed N, Ma X. Stem Cell Secretome, Regeneration, and Clinical Translation: A Narrative Review. Ann Transl Med 2021;9:70. 3). Loi F, Córdova LA, Pajarinen J, et al. Inflammation, fracture and bone repair. Bone. 2016;86:119–130.4). Ridiandries A, Tan JTM, Bursill CA. The Role of Chemokines in Wound Healing. Int J Mol Sci 2018;19:3217 5). Biguetti CC, Cavalla F, Silveira EM et al. Oral implant osseointegration model in C57Bl/6mice: microtomographic, histological, histomorphometric and molecular characterization. J Appl Oral Sci 2018;26:e20170601.