[13p-PB5-1~9] 12.7 Biomedical Engineering and Biochips

[13p-PB5-1] Point-of-care chips using molecularly imprinted polymer nano particle and fluorescent labeled target molecule

〇Shinichi Taniguchi¹, Nobuo Murase², Yukiya Kitayama², Toshifumi Takeuchi² (1.Hitachi, Ltd., 2.Kobe Univ.)

Keywords:molecularly imprinted polymer,point-of-care chips,fluorescent label

Point-of-care (POC) diagnostic tests should be fast, highly sensitive, and affordable. Consequently, bio-mimetic systems for recognizing target molecules are attracting significant attention. Compared with protein antibodies, molecularly imprinted polymers (MIPs), have enormous potential for reducing the production cost of POC tests. Moreover, molecular imprinting is a promising approach for the preparation of synthetic functional polymers capable of the molecular recognition of desirable target molecules. In this presentation, we will report on POC chip prototypes for sensing cortisol (a chronic stress-related hormone) using fluorescent labeled cortisol on conjugate pad and cortisol-imprinted nanoparticles on membrane. The limit of detection for cortisol in POC applications and optimized additives for MIP chip sensing will be discussed.