[14p-1F-1~11] Frontier of Plasma Medicine

[14p-1F-7] Novel Mechanism Gene Transfection Using Non-Equilibrium Atmospheric Pressure Plasma Stimulations

〇Toshiro Kaneko¹, Shota Sasaki¹, Yutaro Hokari¹, Makoto Kanzaki² (1.Dept. of Electronic Eng., Tohoku Univ., 2.Dept. of Medical Eng., Tohoku Univ.)

Keywords:Non-Equilibrium Atmospheric Pressure Plasma,Gene Transfection,Plasma Stimulation

Non-equilibrium atmospheric pressure plasma irradiated to the living-cell is investigated for medical applications such as minimally-invasive and highly-efficient gene transfection. Cell membrane permeability is found to be enhanced by the shorter distance between the solution surface and the adherent cells. This result indicates that the physical stimulation such as the electric field caused by the charged particles arriving at the solution surface, and chemical stimulation associated with plasma-activated products in solution act synergistically to enhance the cell-membrane transport with low-damage.