[16p-2C-1~10] 4.2 Bio- and Medical Photonics

▲ [16p-2C-5] SERS immunosensor for detecting influenza A

〇(D)Kullavadee Karnorachai^1,2, Kenji Sakamoto¹, Rawiwan Laocharoensuk³, Suwussa Bamrungsap³, Tararaj Dharakul^3,4, Kazushi Miki¹ (1.NIMS, Tsukuba, Japan, 2.Tsukuba Univ., 3.NANOTEC, NSTDA, 4.Mahidol Univ.)

Keywords:Biosensor,SERS

Surface-enhanced Raman spectroscopy (SERS) has great promise as a technique for detecting trace amounts of biological and chemical samples, because of its advantages, e.g. nondestructive, no photobleaching, sensitive and fast detection. Our SERS immunosensor is composed of a Au@Ag two-dimensional (2D) array substrate and SERS probes, which are 4, 4’-thiobisbenzenethiol (TBBT)-labeled AuNPs conjugated with antibodies. The detection of influenza A nucleoproteins is achieved by observing the Raman signal of TBBT. We have succeeded in selective detection of influenza A nucleoproteins. We found that the detection sensitivity is increased by using Au@Ag2D array substrate, instead of a SERS inactive substrate (Au film). This improvement is attributed to the plasmonic coupling between the SERS substrate and SERS probes.