11:15 AM - 11:30 AM
▼ [14a-B10-9] Pressure-free nanoimprinting for LSPR biosensor substrate fabrication and application as an immunoassay chip in human IgA detection
Keywords:nano-biosensor, nanoimprint, LSPR
Researches on exquisite nanoparticle or nanostructure for LSPR nanobiosensors have been conducted for over decades due to their superior immediacy in detection, versatility in target analyte, and high potential in sensitivity which make them ideal for POCT (Point-of-care testing) applications. A metallic nano-surface is conventionally fabricated using EBL; however, this limits the POCT suitability since aside from being high-cost, poor performance for large working area and unadaptable to flexible morphology design remain as challenges. Hence, in this work a novel nanoimprinting-based, tunable, easy to scale up and possible of mass-production at low-cost method will be demonstrated. This is considered as a game-changing method as it’s capable of being performed at room-temperature and with no requirement of pressing force. Merits mentioned above fitly meet the needs of biosensor development for POCT application standards.
In this work, absorbance peak caused by LSPR has been confirm at around 530nm wavelength for 50nm Au-sputtered chip. Immunoassay application was done by modifying the metallic surface with Anti-IgA molecule. From the negative control sample (CRP100ng/ml), the peak barely shifted after antigen delivery, while a positive specimen (100ng/ml IgA) triggered a remarkable absorbance shift due to specific antigen-antibody binding events.
In this work, absorbance peak caused by LSPR has been confirm at around 530nm wavelength for 50nm Au-sputtered chip. Immunoassay application was done by modifying the metallic surface with Anti-IgA molecule. From the negative control sample (CRP100ng/ml), the peak barely shifted after antigen delivery, while a positive specimen (100ng/ml IgA) triggered a remarkable absorbance shift due to specific antigen-antibody binding events.