3:45 PM - 4:15 PM
[14p-A23-5] Biocompatibility of Diamond like Carbon and its bio-application
Keywords:DLC, Biocompatibility, Biomaterial
Diamond-Like Carbon (DLC) films have excellent properties such as hemocompatibility, biocompatibility, and in-vivo stability, offering a means of modifying surface for medical devices. However, the properties of DLC films strongly depend on coating conditions, fabrication equipments and deposition methods. Therefore, to produce a required film, it has to be carefully selected a manufacture method and a coating condition. It was found that conventional studies suggested strong correlation between cell adhesion and C=O bonds on sample' surface. In general, samples are fixed in vacuum for X-ray photoelectron spectroscopy (XPS), which gives quantitative analysis of bonding condition of a DLC film surface. Practical applications of DLC-coated medical device require more simplified evaluation method. In this study, we have estimated the relationship between cell adhesion and optical properties of DLC films by focusing refraction index and extinction coefficient using spectral ellipsometry measurements.
Various DLC films (32 samples) were coated on polystyerene (PS) and stainless steel (SUS316L) dishes by chemical vapor deposition methods and physical vapor deposition methods. The cell culture was carried out for 72 hours using mouse fibroblast (NIH-3T3). Additionally, the surface conditions of DLC films were estimated by XPS, contact angle measurement and spectral ellipsometry measurements. From this result, the majority of DLC films is classified as Polymer-like Carbon (PLC) and a-C (Typical classofocation of DLC films) are the enhancement types of cell adhesion. It was found that the relationship between cell adhesion and optical properties (refraction index and extinction coefficient) of DLC films. DLC films are categorized by the refraction index and extinction coefficient because biocompatitibility is strongly depended on their optical properties can evaluate cell adhesion of DLC films easily.
Various DLC films (32 samples) were coated on polystyerene (PS) and stainless steel (SUS316L) dishes by chemical vapor deposition methods and physical vapor deposition methods. The cell culture was carried out for 72 hours using mouse fibroblast (NIH-3T3). Additionally, the surface conditions of DLC films were estimated by XPS, contact angle measurement and spectral ellipsometry measurements. From this result, the majority of DLC films is classified as Polymer-like Carbon (PLC) and a-C (Typical classofocation of DLC films) are the enhancement types of cell adhesion. It was found that the relationship between cell adhesion and optical properties (refraction index and extinction coefficient) of DLC films. DLC films are categorized by the refraction index and extinction coefficient because biocompatitibility is strongly depended on their optical properties can evaluate cell adhesion of DLC films easily.