[14p-C42-1~21] 3.4 Biomedical optics

[14p-C42-7] In vivo imaging of hemodynamics during hepatic ischemia-reperfusion in rats based on spectrocolorimetry

Sharmin Akter¹, Satoko Kawauchi², Shunichi Sato², Suefumi Aosasa³, Junji Yamamoto³, 〇Izumi Nishidate¹ (1.Tokyo Univ. Agri. Tech. BASE, 2.National Defense Medical College Research Institute, 3.National Defense Medical College)

Keywords:Diffuse reflectance spectroscopy, Hepatic ischemia-reperfusion, Imaging of hemodynamics

In the present study, we investigate a method to monitor the spatial distribution of concentrations of oxygenated hemoglobin C_HbO, deoxygenated hemoglobin C_HbR, total hemoglobin C_HbT, and tissue oxygen saturation StO₂ in hepatic tissue using a digital red-green-blue camera. The experimental results with in vivo rat hepatic ischemia-reperfusion model indicate that the method applied in the present study is reliable and has potential application in the intraoperative assessment of graft tissue viability.