[14p-P19-1~20] 12.7 Biomedical Engineering and Biochips

[14p-P19-7] Direct Electron Transfer between Single-Walled Carbon Nanotube and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase

〇hiroki hidaka¹, hitoshi muguruma¹, hisanori iwasa², atsunori hiratsuka², hirotaka uzawa² (1.Shibaura Inst., 2.National Inst.)

Keywords:Carbon nanotubes, FAD-GDH, Sensor

Mediatorless direct electron transfer (DET) between flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) and single-walled carbon nanotube (SWNT) is presented. The enzyme electrode is formed by a simple layer-by-layer process in which a droplet of SWCNT/surfactant solution onto a current collector (Au surface) and allow it to evaporate, subsequently a droplet of enzyme solution onto SWCNT layer. In cyclicvoltammetry, the large current output (1.5 mA/cm²) due to 48 mM glucose at more than +0.4V at FAD-GDH/SWNT/surfactant electrode is observed. Therefore, DET between FAD-GDH and SWCNT is accomplished. The detail of DET is discussed with the recently reported crystallographic model of FAD-GDH.