[14p-P23-1~11] English session: Joint symposium on Nanobiotechnology and Biosensing

▲ [14p-P23-9] Organic and Inorganic Nanocomposite Structure Using Protein Moleculars

〇Tyou Ka¹, Mutsunori Uenuma¹, Naofumi Okamoto¹, Ichiro Yamashita¹, Yukiharu Uraoka¹ (1.NAIST)

Keywords:Bio Nano Process, ferritin, nanocomposite structure

To achieve nanoscale controlled structure, Bio Nano Process (BNP) has been proposed [1]. In the BNP, ferritin, which is consisted of 24 polypeptide subunits, is used to make nanocomposite structure. The inner and outer diameters of the ferritin protein shell are about 7 nm and 12 nm. We have been developing a process to make two dimensional array of ferritin on a surface [2]. On the other hand, introducing nanostructures is a novel way to optimize the thermoelectric properties for decreasing thermal conductivity. However, fabrication of fine nanostructures is still hard to be realized. Therefore, we have utilized BNP to fabricate organic nanostructure (low thermal conductivity) in inorganic materials. Iron-core ferritin and apo-ferritin were used to fabricate nanostructure in organic materials (silicon oxide). The process detail is that, at first, the samples were treated by UV-Ozone for 10 min at 115^oC. Then, the ferritins were drop on the samples and spin coated. Finally, the silicon oxide thin film was fabricated on ferritins by electron beam evaporation. In order to evaluate the nanostructure, cross-sectional TEM image has been taken. From TEM image (Fig. 1), nanocomposite structure of ferritin within inorganic nanoparticles embedded in silicon oxide film (Fig. 1a) and nanostructure of apo-ferritin only embedded in silicon oxide film (Fig. 1b) can be observed, respectively. Therefore, nanocomposite structures with local nanosize protein spots were successfully fabricated by BNP.