[15a-P1-1~10] 3.4 Biomedical optics

[15a-P1-8] Analysis of Quantum Dots Using Fast-Fluorescence Correlation Spectroscopy

〇(M2)Makoto Oura¹, Yamamoto Johtaro², Kinjo Masataka² (1.Hokkaido Univ. Life, 2.Hokkaido Univ. Adv. Life)

Keywords:Optic microscopy, Spectroscopy, Quantum dot

Fluorescence correlation spectroscopy (FCS) is one of the spectroscopy technique using confocal laser optic system to analyze the molecular concentration and translational diffusional dynamics in solution and in living cells. It allows to measure including fluorescent molecules, quantum dots (QDs) and gold nanoparticles (GNPs) to estimate their hydrodynamic radius or photochemical properties. Here, we used two avalanche photodiodes (APDs) and a high-speed hardware correlator in our detection system to get fast correlation fraction up to 1.56 ns. Using this instrument, we measured pH-depended blinking of QDs and lifetime-depended fraction (antibunching) simaltaneously. It is a powerful method to analyze such nanoparticles in various samples.