[19p-W331-1~16] 12.7 Biomedical Engineering and Biochips

[19p-W331-12] Sensitive Immunosensing with a Sandwich Assay on the ZnO-coated Plasmonic Chip

〇Keiko Tawa¹, Masashi Sumiya¹, Sasakawa Chisato¹, Takuma Sujino², Hikaru Nakazawa², Mitsuo Umetsu² (1.Kwansei Gakuin Univ., 2.Tohoku Univ.)

Keywords:Plasmon,Bispecific antibody,Sandwich assay

The grating substrate covered with a thin metal films is called as a plasmonic chip in which the incident light can be directly coupled with surface plasmon polaritons at the resonance angle. The enhanced electric field excites fluorescent molecules and bright fluorescence was obtained due to the grating-coupled surface plasmon field-enhanced fluorescence (GC-SPF). In this study, IL-6 was sensitively detected on the ZnO-coated plasmonic chip with a sandwich assay including ZnO x IL-6 bispecific antibody as a capture antibody and alexa-labeled anti IL-6 antibody as a detection antibody.