[19p-W331-1~16] 12.7 Biomedical Engineering and Biochips

[19p-W331-13] Limit of detection (LOD) of point-of-care chips using molecularly imprinted polymer beads

〇Shinichi Taniguchi¹, Nobuo Murase², Yukiya Kitayama², Toshifumi Takeuchi² (1.Hitachi, Ltd., 2.Kobe Univ.)

Keywords:molecularly imprinted polymer,Point-of-care,Chip

Point-of-care (POC) diagnostic tests should be fast, highly sensitive, and affordable. Consequently, bio-mimetic systems for recognizing target molecules are attracting significant attention. Compared with protein antibodies, molecularly imprinted polymers (MIPs), have enormous potential for reducing the production cost of POC tests. Moreover, molecular imprinting is a promising approach for the preparation of synthetic functional polymers capable of the molecular recognition of desirable target molecules. In this presentation, we will report on POC chip prototypes for sensing cortisol (a chronic stress-related hormone) using fluorescent labeled cortisol on conjugate pad and cortisol-imprinted nanoparticles on membrane.
The fluorescent intensities at the test line and conjugate pad before and after the dummy sample addition were compared. After sample addition, the intensity at the conjugate pad decreased, and that at the test line increased. These results indicate MIP beads have captured fluorescent cortisol and suggest the limit of detection (LOD) of the chip is under 250 nmol/L of cortisol. This chip has great potential to be applied to a saliva test. The detailed properties of the imprinted polymers that allow recognition of the target hormone and utility of MIPs with fluorescence to detect the target hormone in POC applications will be discussed.