[20a-W331-1~12] 12.7 Biomedical Engineering and Biochips

△ [20a-W331-11] Development of H₂O₂ sensor for GABA and Glutamate imaging

〇(M1)Yuki Okumura¹, Kouichi Okumura^1,2, Tatsuya Iwata^1,2, Makoto Ishida¹, Kazuaki Sawada^1,2 (1.Toyohashi Univ., 2.JST-CREST.)

Keywords:CCD type image sensor,H2O2,imaging

We have been developed the sensor for the imaging of neurotransmitter such as adenosine triphosphate (ATP) and acetylcholine (Ach). In this study, we proposed the sensor for detection of γ-aminobutyric acid (GABA) and L-glutamate (GLU) because these are important as neurotransmitters to adjust excitatory/inhibitory balance in brain. Our proposed sensor has single pixel and based on one enzymatic reactions which are that of H₂O₂. This sensor can determine H₂O₂ concentration as the potential change of gold caused by electrons transfer produced by enzymatic reaction of H₂O₂ derived from that of L-glutamate.