[21a-W331-1~12] 12.7 Biomedical Engineering and Biochips

[21a-W331-3] Rapid immunoassays assisted by thermal convection control

〇Tatsuro Tadokoro¹, Masato Saito¹, Eiichi Tamiya¹ (1.Osaka Univ.)

Keywords:ELISA,thermal convection

Enzyme-Linked ImmunoSorbent Assay (ELISA) is one of the biochemical tests that used to determine the quantity of biomarkers in clinical diagnostics. It requires labor-intensive and time-consuming work, and trained technicians so it is conducted only in hospital and test laboratories. In order to realize on-site ELISA, we developed the microfluidic chip and the rotation device that could control solution flow by the centrifugal force without complicated process using syringe pumps. The solution in the reaction field could be circulated by thermal convection to increase mass transport efficiency. The flow rate was controlled by the rotation speed and the temperature difference of the control device attached to the bottom of the chip. In addition, the chip was designed to exchange the solution by rotation for simple immunoassay operation. The calibration curve for Human IgA was obtained.