[6p-A402-1~11] 3.4 Biomedical optics

[6p-A402-4] Valuation of damage on living cells by short wavelength visible laser beam

〇Keisuke Takahashi¹, Syohei Kawahara¹, Naohiro Kanamaru¹, Tetsuya Matsuyama¹, Kenji Wada¹, Ai Kawakita², Kaori Murata², Kenji Sugimoto² (1.Engineering Osaka Pref Univ., 2.Life and Environmental Sciences Osaka Pref Univ.)

Keywords:live cell imaging, laser, phototoxicity

We need to clarify the generating mechanism of the damage caused by the irradiation of blue light. In this study, we investigate the effect of short-wavelength visible laser irradiation to cell nucleus and cytoplasm of living cells under the condition that the excitation light for fluorescent proteins has no influence on the cell viability and mitotic progression. In the case of irradiation energy of 72, 96 kJ/cm², irradiation to the cell nucleus has more harmful than that to the cytoplasm, whereas the laser irradiation of 144, 168 kJ/cm² to cell nucleus and cytoplasm cause the cell death with same probability.