[15p-313-1~10] English session: Joint Symposium on Nanobiotechnology and Biosensing

▲ [15p-313-7] Fabrication of Au-nanoparticle-embedded lipid bilayers on solid substrates

Naotoshi Sakaguchi¹, Kimura Yasuo², Ayumi Hirano-Iwata³, 〇Toshio Ogino¹ (1.Yokohama Natl. Univ., 2.Tokyou Univ. Technol., 3.Tohoku Univ.)

Keywords:lipid bilayer, metal nanoparticle, biodevice

Au-nanoparticles (Au-NPs) were incorporated into lipid vesicles by hydrophobization of the Au-NP surfaces. Au-NP-embedded lipid bilayers supported on solid surfaces were fabricated by the vesicle-vesicle fusion, in which the Au-NP-embedded vesicles are adsorbed to the substrate, ruptured and fused, or by the vesicle-membrane fusion, in which the Au-NP-embedded vesicles are fused into preformed Au-NP-free supported lipid bilayers. The Au-NP incorporation was observed by atomic force microscopy (AFM) and transmission electron microscopy. The Au-NPs form two-dimensional particle sheets with a uniform particle density in lipid bilayers. We found that vesicle approach control by addition of charged lipids and destabilization of the lipid bilayers by addition of lipids with a small polar head group are key techniques in the vesicle-membrane fusion.