2017年第64回応用物理学会春季学術講演会

講演情報

シンポジウム(口頭講演)

シンポジウム » ナノバイオテクノロジーとバイオセンシングに関するジョイントシンポジウム

[15p-313-1~10] ナノバイオテクノロジーとバイオセンシングに関するジョイントシンポジウム

2017年3月15日(水) 13:15 〜 18:30 313 (313+314)

竹原 宏明(東大)、平野 愛弓(東北大)、宮本 浩一郎(東北大)

16:30 〜 16:45

[15p-313-7] Fabrication of Au-nanoparticle-embedded lipid bilayers on solid substrates

坂口 直駿1、木村 康男2、平野 愛弓3、〇荻野 俊郎1 (1.横浜国大、2.東京工科大、3.東北大)

キーワード:lipid bilayer, metal nanoparticle, biodevice

Au-nanoparticles (Au-NPs) were incorporated into lipid vesicles by hydrophobization of the Au-NP surfaces. Au-NP-embedded lipid bilayers supported on solid surfaces were fabricated by the vesicle-vesicle fusion, in which the Au-NP-embedded vesicles are adsorbed to the substrate, ruptured and fused, or by the vesicle-membrane fusion, in which the Au-NP-embedded vesicles are fused into preformed Au-NP-free supported lipid bilayers. The Au-NP incorporation was observed by atomic force microscopy (AFM) and transmission electron microscopy. The Au-NPs form two-dimensional particle sheets with a uniform particle density in lipid bilayers. We found that vesicle approach control by addition of charged lipids and destabilization of the lipid bilayers by addition of lipids with a small polar head group are key techniques in the vesicle-membrane fusion.