[21a-144-1~13] 8.4 Plasma life sciences

△ [21a-144-3] Investigation of Mechanism for Drug Simulated Molecule Introduction Induced by In-Liquid Plasma

〇(M2)Ryosuke Honda¹, Shota Sasaki¹, Keisuke Takashima¹, Makoto Kanzaki², Takehiko Sato³, Toshiro Kaneko¹ (1.Graduate School of Engineering, Tohoku Univ., 2.Graduate School of Medical Engineering, Tohoku Univ., 3.Institution of Fluid Science, Tohoku Univ.)

Keywords:in-liquid plasma

We conducted experiments to search of the drug simulated molecules introduction pathway in cells for developing of in-liquid plasma generation device assuming a local and minimally invasive gene or drug molecule introduction technique in the human body. The YOYO-1 introduction was increased by in-liquid plasma treatment and Ruthenium Red addition significantly inhibited the YOYO-1 introduction effect by in-liquid plasma. Ruthenium Red, RR inhibit a cation-permeable channels on cell membrane. It was assumed that the plasma-induced YOYO-1 introduction might be realized by not membrane damage but cellular responses mediated by cation-permeable channels.