[19p-A302-1~23] 3.4 Biomedical optics

[19p-A302-6] Fluorescence-Lifetime-Surpassed Ultrafast Confocal Fluorescence Microscopy

〇Hideharu Mikami¹, Yasuyuki Ozeki², Keisuke Goda^1,3 (1.School of Sci., Univ. of Tokyo, 2.School of Eng.Univ. of Tokyo, 3.JST)

Keywords:confocal fluorescence microscopy, 3D imaging, imaging flow cytometry

We present a confocal fluorescence microscope that surpasses the highest possible frame rate constrained only by the fluorescence lifetime of fluorophores, which is enabled by telecommunication technologies such as frequency-division multiplexing (FDM) and quadrature amplitude modulation (QAM). We implemented the FDM and QAM using an interferometer with acousto-optic modulators and polarization optical components. To show its broad utility, we demonstrated 3D volumetric confocal fluorescence microscopy of cellular dynamics at 104 volumes/s and high-throughput imaging flow cytometry.