[19p-PB1-1~34] 12.7 Biomedical Engineering and Biochips

▲ [19p-PB1-28] Modified Centrifugal Microfluidic Chip System with Reflow Capability for Single Cell Preparation

〇(PC)Wilfred Espulgar¹, Masato Saito¹, Shohei Koyama¹, Hyota Takamatsu¹, Atsushi Kumanogoh¹, Eiichi Tamiya¹ (1.Osaka University)

Keywords:microfluidics, single cell, reflow

A reflow system based on the reverse rotation of a centrifugal chip has been reported previously by our group. It allows the cell suspension to simply interact with the hydrodynamic traps several times which could provide a higher probability of trapping. This enables the decrease in the number of wasted cells in a suspension perfused through a typical microfluidic chip. Microfluidics technology has proven its usefulness in preparing single cells for Life Science Research. In our previous design, a trapping efficiency of 55% was obtained using THP-1 cells. Also, the minimum operation of the device was at 800 rpm that can be damaging or harmful to cells. With this in mind, the improved design was sought to be operated at lower rpm and with higher trapping efficiency.