[19p-PB1-1~34] 12.7 Biomedical Engineering and Biochips

[19p-PB1-6] Binding activity of sialyllactosamine derivatized on BSA to influenza viruses

〇Toshio Kawahara¹, Hiroaki Hiramatsu¹, Yuhsuke Ohmi¹, Yasuo Suzuki¹, Kyoko Hayashi¹, Shin-ichi Nakakita², Yohei Watanabe³, Yasuhide Ohno⁴, Kenzo Maehashi⁵, Takao Ono⁶, Yasushi Kanai⁶, Kazuhiko Matsumoto⁶ (1.Chubu Univ., 2.Kagawa Univ., 3.KPUM, 4.Tokushima Univ., 5.TUAT, 6.Osaka Univ.)

Keywords:influenza virus, sugar chain, binding activity

For biosensors with host specificity for influenza viruses, we used sugar chains with different coordinations of terminal sialic acid and penultimate galactose. Sialyllactosamine (SiaLacNAc) is one of the candidates for detecting molecules in the biosensors for influenza viruses, and we have investigated the host specificity of the viruses using an enzyme-linked immunosorbent assay (ELISA). Using the bovine serum albumin (BSA) localization, we have obtained the stable distribution. The clustering of sugar chains could also enhance and stabilize the binding activity.