[19p-PB1-1~34] 12.7 Biomedical Engineering and Biochips

[19p-PB1-7] Development of deoxyribonuclease sensors using electrical properties of DNA

Shota Tsukamoto¹, 〇Takahiro Himuro¹, Yoji Saito¹ (1.Seikei Univ.)

Keywords:DNA, Deoxyribonuclease, Biosensor

We newly fabricated a novel microdevice for detection of deoxyribonuclease (DNase), enzyme for nonspecific DNA cleavage, which is a candidate biomarker for acute myocardial infarction. Multiple λDNA molecules were immobilized between two electrodes in a microchannel, and DNase detection was achieved by measuring the increase in impedance between the electrodes after DNA cleavage by DNase.