[10a-M116-1~8] 3.1 Basic optics and frontier of optics

[10a-M116-2] Subtraction Imaging using Higher-Order Vector Beams for Biological Samples

Mio Yoshida¹, 〇Yuichi Kozawa¹, Shunichi Sato¹ (1.IMRAM, Tohoku Univ.)

Keywords:Vector beam, Confocal laser scanning microscopy, Super-resolution imaging

We develop a new method that can improve the spatial resolution in confocal laser scanning microscopy (CLSM) simply by subtracting two images acquired by bright- and dark-spot-shaped point spread functions. We have previously demonstrated that the usage of higher-order vector beams such as radially and azimuthally polarized beams in subtraction imaging can dramatically improve the spatial resolution in CLSM with reduced negative side-lobe generation. In this study, we apply our method to biological samples and evaluate its imaging capability in terms of the impact of negative side-lobe generation.