[10p-PA3-1~21] 12.7 Biomedical Engineering and Biochips

[10p-PA3-1] Antibody-fused molecularly imprinted polymers capable of exosome fluorescence sensing for cancer diagnosis

〇Kisho Mori¹, Wataru Katayama¹, Taku Shimizu¹, Kazuaki Minamoto¹, Eri Takano¹, Yukiya Kitayama¹, Toshifumi Takeuchi¹ (1.Grad. Sch. Eng., Kobe Univ.)

Keywords:exosome, Molecularly imprinted polymers, Antibodies

Exosomes are secreted from various cells and contain proteins, microRNAs and mRNAs. They have been found to play important roles in intercellular communications, and may be deeply involved in the pathogenesis of neurodegenerative diseases and the microenvironment formation for metastasis of cancers. In this study, antibody-fused molecularly imprinted polymer (MIP) thin films for detecting intact exosomes were prepared, where the in-cavity introduction of fluorescent probes was conducted via post-imprinting modification, which enables highly sensitive fluorescence detection of intact exosomes without pretreatment such as ultracentrifugation. Exosomes originating from the cancer cells were successfully detected using variety of antibodies introduced in the developed sensing materials.