[11p-M121-1~10] Interplay and integration of experiments and calculations in nanobiotechnology: lipid membrane and membrane proteins

[11p-M121-5] Formation of artificial lipid bilayer on surface modified substrate and application for the observation of reconstituted membrane proteins

〇Toshinori Motegi^1,2, Hiroyuki Hoshino¹, Kota Sakamoto³, Fumio Hayashi³, Masashi Sonoyama^1,2 (1.Fac. Sci. Tech., Gunma Univ., 2.Ctr. Food Sci. and Wellness, Gunma Univ., 3.Ctr. Inst. Anal., Gunma Univ.)

Keywords:lipid bilayer membrane, membrane protein, atomic force microscopy

Two-dimensional lipid bilayer on the solid substrate (SLB) has been useful for understanding the function and structure of membrane protein, but it has possibility of structural deformation and random orientation due to the contact with substrate. In this study, self-assembled monolayer (SAM) that binds to the specific site of membrane protein was formed on flat mica and SLB was formed thereon. Lower interaction between SLB and substrate bring decreased diffusion of intra-membrane dye-lipids obtained from FRAP method with fluorescence microscope. Light driven proton pumps with different size of extra- and intracellular region was successfully reconstructed into SLB, and evaluated their orientation by AFM. The established basic technique that reduces structural deformation and inactivation should enable structure analysis of membrane proteins of specific orientation and reaction analysis with other molecules.