[9p-W241-1~12] 8.4 Plasma life sciences

[9p-W241-1] Observation of HeLa cells incubated in plasma treated solutions

Shogo Maeda¹, Yugo Hosoi¹, 〇Kenji Ishikawa¹, Hiroshi Hashizume¹, Hiromasa Tanaka¹, Masaru Hori¹ (1.Nagoya Univ.)

Keywords:Plasma activated lactate, Antitumor effect, holotomography

Ringer’s lactate solution (Lactec) is used for intravenous injection. Plasma-activated lactec (PAL) for the selective killing of cancer cells, was reported. Intracellular responses interacted with the plasma-activated liquid have been recently studied. However, mechanisms of killing effects of cancer cells by the PAL have not been elucidated comprehensively. In the case of PAL, intracellular reactive nitrogen and oxygen species (RONS) such as H₂O₂ and NO₂⁻ plays an important role for apoptotic death of cancer cells. Thus the intracellular oxidative stress has been attracted much attentions in the PAL treatments. On contrast, we found that PAL included plasma-activated organic species, generated by reacting lactic acid in the Lactec with plasma. In this study, we have investigated individual components of the PAL and intracellular RONS of HeLa cells observed by microscopy.