[9p-W241-1~12] 8.4 Plasma life sciences

△ [9p-W241-6] Explain molecular size dependence of gene transfer efficiency in surface discharge with collision frequency between cell membranes and introduced molecules.

〇(M1)Tatsuya Hiramatsu¹, Hiroko Hirashige¹, Yugo Kido^1,2, Susumu Satoh^1,3, Masahumi Jinno¹ (1.Ehime.Univ, 2.Pearl Kogyo Co.,Ltd, 3.Y's Corp.)

Keywords:Plasma, Gene transfection, Molecular size

Currently, various gene transfection methods are put to practical use. Among them, The gene / molecule introduction method using surface discharge enables a wide range of gene introduction with a single treatment, and it is possible to efficiently obtain the transferred cells. In this research, we tried to clarify the molecular size dependence of the transfection efficiency in surface discharge method as part of clarification of the transfection mechanism for practical application. Furthermore, the relationship between molecular weight of DNA and collision frequency of DNA molecule and cell membrane was calculated and the effect of the molecular weight on introduction was discussed.