10:15 AM - 10:30 AM
[12a-A408-6] Detection of fibrillization of α-Synuclein by liposome-immobilized Quartz-CrystalMicrobalance Mechanical Sensor
Keywords:biosensor, alpha-synclein, QCM
α-synuclein (αSyn) has been majorly recognized as causative agent for Parkinson Disease (PD). Its monomer usually existing in our brain cell starts to aggregate and grow as oligomers, protofibrils and fibrils, which are recognized to be toxic. Therefore, the detection of the αSyn fibril is essential for early diagnosis of PD.
We have so far developed and reported label-free liposome-immobilized cantilever sensor in order to obtain a trace amount of chronological behavior of αSyn, realizing its fibrillar detection as small as 700 fM, which showed nearly the same detectivity as recent ELISA’s. This time, we proceeded to develop and apply another type of mechanical sensor of Quartz-Crystal-Microbalance (QCM) for the detection. Finally, we successfully detected the adsorption and successive fibrillization of αSyn fibril on the Au surface of QCM oscillator down to 7 pM, suggesting that label-free liposome-immobilized QCM is available for the detection of αSyn fibril.bai
We have so far developed and reported label-free liposome-immobilized cantilever sensor in order to obtain a trace amount of chronological behavior of αSyn, realizing its fibrillar detection as small as 700 fM, which showed nearly the same detectivity as recent ELISA’s. This time, we proceeded to develop and apply another type of mechanical sensor of Quartz-Crystal-Microbalance (QCM) for the detection. Finally, we successfully detected the adsorption and successive fibrillization of αSyn fibril on the Au surface of QCM oscillator down to 7 pM, suggesting that label-free liposome-immobilized QCM is available for the detection of αSyn fibril.bai