[12p-PA6-1~15] 12.6 Nanobiotechnology

[12p-PA6-7] Arrangement of vesicles to lipid bilayer suspended over microwells using biotin-avidin binding

Yuto Nakatani¹, Ami Yasuhara¹, Azusa Oshima², Yuko Ueno², Akira Heya¹, 〇Koji Sumitomo¹ (1.Univ. of Hyogo, 2.NTT BRL)

Keywords:nanobiodevice, lipid bilayer, control of arrangement

In the Lα/Lo phase separated lipid membrane on the microwell substrate, the Lα phase lipid aggregates on the freestanding membrane over microwells. On the other hand, biotin-modified lipids are also localized in the Lα phase domain. We propose a method to arrange vesicles directly to freestanding lipid membranes using biotin-avidin interaction. It can also be expected to be applied to technology for controlling the arrangement of membrane proteins etc. on the nanobiodevice with a microwell substrate.