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▲ [12a-S402-3] Intracellular cargo delivery through electrochemical metal-organic hybrid nanogate
Keywords:metal-organic hybrid nanogate
Intracellular cargo delivery plays an important role in fundamental biological research and therapeutic medical applications. The cargo delivery requires safe and efficient access to cells and specifically different intracellular locations due to impermeable outer cell membranes. Nanostructures are a promising candidate for membrane disruption, through which versatile cargos transport into cells to overcome impermeable plasma membrane. Hollow nanostructures combined with electroporation are potentially valuable in interdisciplinary fields owing to their ability to transport versatile cargos into adhesive cells. However, they require voltages over 1.5 V to electroporate the physical barrier of the cell membrane inducing cell death and differentiation processes. Here we have developed the PEDOT/Au hybrid NT stamping with high efficient and precise dosage control by the small external voltage (Fig.1). The hybrid stamp improved the cell viability by over 94 % for 30 min physical insertion while decreased to less than 1% using original Au NTs. Furthermore, the hybrid stamp acted as an electrochemical gate that can open the pore at ±50 mV to transport small molecules of calcein dye with high efficiency (99%) and high viability (96.8 %). The hybrid nanogate can also transport large molecules of a GFP protein with 84% efficiency and 98.5% viability and GFP plasmid at a transfection rate of approximately 10%. Thus, the present hybrid stamping can potentially deliver versatile molecules into adhesive cells with high efficiency and viability.