[13a-N202-1~12] 3.4 Biomedical optics

△ [13a-N202-10] Four-wave mixing and second-harmonic generation imaging flow cytometry

〇(DC)Ryo Kinegawa¹, Julia Gala De Pablo¹, Wang Yi², Hiramatsu Kotaro^1,3,4, Goda Keisuke^1,5,6 (1.Department of Chemistry, The University of Tokyo, 2.Department of Chemistry, Renmin University of China, 3.Research Centre for Spectrochemistry, The University of Tokyo, 4.PRESTO, Japan Science and Technology Agency, 5.Institute of Technological Sciences, Wuhan University, 6.Department of Bioengineering, University of California)

Keywords:imaging flow cytometry, four-wave mixing, second-harmonic generation

We demonstrate the world’s first four-wave mixing (FWM) and second-harmonic generation (SHG) imaging flow cytometry, enabling us to obtain a statistical number (N > 1,000) of single cells’ morphological information and to visualize non-centrosymmetric media in cells in a label-free manner. Our FWM and SHG imaging flow cytometer is composed of an FWM and SHG microscope with a 2D-rapid galvanometric scanner, a microfluidic device, and a high-speed image acquisition circuit, enabling a frame rate of 10 fps with a pixel resolution of 100 × 100 pixels. As a proof-of-principle demonstration, we used the FWM and SHG imaging flow cytometer to perform FWM and SHG imaging of polymer beads and Chromochloris zofingiensis (NIES-2175) cells. Our FWM and SHG flow cytometer paves the way for statistical studies of cellular dynamics and drug screening in a label-free manner.