15:15 〜 15:30
▲ [13p-N404-6] 3D Glass Microfluidic SERS Chip Fabricated by Hybrid Femtosecond Laser Processing for Biomolecule Sensing
キーワード：Laser processing, Microfluidic chip, SERS
Microfluidic surface-enhanced Raman scattering (SERS) chip is increasingly becoming an attractive tool for biomedical sensing, due to its high sensitivity, repeatability, in-situ and real-time analysis, which includes cancer identification, tissue therapy, and bio-imaging.[1] Recently, we developed a novel technique termed liquid-interface assisted SERS (LI-SERS) which realized attomolar sensing with an enhancement factor of 1.5 × 1014.(Fig. 1 (a)) [2] The microfluidic SERS chips were fabricated using hybrid femtosecond laser processing consisting of femtosecond laser assisted chemical etching, selective metallization and metal surface nanostructuring.[3] The nanostructuring of Ag/Cu SERS substrate in the microfluidic chip was performed based on 515 nm femtosecond laser induced periodic surface structure (fs-LIPSS). The enhancement mechanisms for LI-SERS were ascribed to the laser-induced local aggregation and optical trapping of analyte molecules on SERS substrate. In this study, we demonstrate the applications of LI-SERS using microfluidic SERS chips for biomolecule sensing, including the discrimination of deoxyribonucleic acid sequences (DNA oligo) (Fig. 2(b)) and the detection of proteins.
Figure 1. (a) The time-lapse intensity change at 1183 cm-1 for R6G solution (10-11 M) by LI-SERS using the microfluidic SERS chip. The intensity drastically changes depending on the excitation laser irradiation schemes on SERS substrate which are (Ⅰ) in analyte solution, (Ⅱ) at the interface, and (Ⅲ) in air. (b) Discrimination of DNA oligos (10-12 M) using LI-SERS method.
References
[1] S. Bai, K. Sugioka. Light Adv. Manuf. 2021, 2, 1.
[2] S. Bai, D. Serien et al. ACS Appl. Mater. Inter. 2020, 12, 42328.
[3] S. Bai, D. Serien et al. Adv. Funct. Mater. 2018, 28, 1706262.
Figure 1. (a) The time-lapse intensity change at 1183 cm-1 for R6G solution (10-11 M) by LI-SERS using the microfluidic SERS chip. The intensity drastically changes depending on the excitation laser irradiation schemes on SERS substrate which are (Ⅰ) in analyte solution, (Ⅱ) at the interface, and (Ⅲ) in air. (b) Discrimination of DNA oligos (10-12 M) using LI-SERS method.
References
[1] S. Bai, K. Sugioka. Light Adv. Manuf. 2021, 2, 1.
[2] S. Bai, D. Serien et al. ACS Appl. Mater. Inter. 2020, 12, 42328.
[3] S. Bai, D. Serien et al. Adv. Funct. Mater. 2018, 28, 1706262.