3:15 PM - 3:30 PM
[16p-Z21-7] Improvement of sensitivity of αSynuclein fibril as causative agent of Parkinson's disease by adding NaCl into buffer solution of liposome sensor
Keywords:Biosensor, Alpha-Synuclein, QCM
α-synuclein (αSyn) has been majorly recognized as causative agent for Parkinson Disease (PD). Its monomer usually existing in our brain cell starts to aggregate and grow as oligomers, protofibrils and fibrils, which are recognized to be toxic. Therefore, the detection of a trace amount of the αSyn fibril is essential for early diagnosis of PD.
Recently, it has been reported that NaCl promotes the aggregation of monomeric αSyn. In this study, we examined whether the aggregation process of αSyn could be detected more efficiently by liposome -immobilized QCM, using NaCl solution as a buffer, compared to PBS one. We added a trace amount of αSyn fibril solution (7 pM) into the NaCl solution in the QCM sensor cell, with the concentration ranging from 0.5 M to 1.0 M. Thereafter, the resonance frequency shift became significantly larger in the buffer solution of NaCl than those of PBS. Also, the sensitivity increased with increasing NaCl concentration from 0.5 M to 1.0 M.
Recently, it has been reported that NaCl promotes the aggregation of monomeric αSyn. In this study, we examined whether the aggregation process of αSyn could be detected more efficiently by liposome -immobilized QCM, using NaCl solution as a buffer, compared to PBS one. We added a trace amount of αSyn fibril solution (7 pM) into the NaCl solution in the QCM sensor cell, with the concentration ranging from 0.5 M to 1.0 M. Thereafter, the resonance frequency shift became significantly larger in the buffer solution of NaCl than those of PBS. Also, the sensitivity increased with increasing NaCl concentration from 0.5 M to 1.0 M.