[19a-Z04-1~12] 3.4 Biomedical optics

[19a-Z04-4] Super-resolution transmission imaging of stained tissue sections by using saturated absorption

〇Hikaru Sato¹, Kentaro Nishida^1,2, Ryosuke Oketani¹, Kentaro Mochizuki³, Kenta Temma^1,2, Yasuaki Kumamoto¹, Hideo Tanaka³, Katsumasa Fujita^1,2 (1.Osaka Univ., 2.AIST Advanced Photo-Bio Lab, 3.Kyoto Prefectural Univ of Medicine)

Keywords:Optical microscopy, Absorption saturation, Super-resolution

In this study, the spatial resolution of a laser scanning transmission microscopy is improved by using saturated absorption of dyes.This phenomenon occurs locally near the center of the illumination spot and generates transmitted signal component that exhibits a non-linear response to the excitation light intensity. By performing imaging while selectively detecting non-linear transmitted signal response, it is possible to compose an image from a transmitted signal generated from a region smaller than the illumination spot. Images of stained tissue sections show that this method improves the spatial resolution of transmission microscopy.