[20a-P06-1~11] 12.7 Biomedical Engineering and Biochips

[20a-P06-6] Monitoring of a PCR process on a Quartz Crystal Microbalance (QCM)-biosensor

〇(B)Hideto Kumagai¹, Hiroyuki Furusawa², Shizuo Tokito¹ (1.Eng., Yamagata Univ., 2.IPGE, Yamagata Univ.)

Keywords:quartz crystal microbalance, DNA isothermal amplification, PCR monitoring

In recent years, a PCR method has been attracting attention due to a test for viral infection. Generally, PCR products are detected by using a fluorescent labeling agent and an optical device. In this study, a QCM mass sensor was investigated to be another method to monitor PCR products. We performed a PCR reaction on a QCM plate on which a primer was immobilized with monitoring of the QCM signal and then conformed it using electrophoresis. The mass increase was observed from the QCM response when a PCR reaction progressed. We hope that QCM could become a handy-sized device and one of the devices for a PCR test.