[22p-A106-1~15] 12.6 Nanobiotechnology

[22p-A106-3] Detection of a single exosome using two types of membrane protein on a plasmonic chip by the transmitted-light fluorescence microscopy

〇(M2)Kazuma Fukutomi¹, Keiko Tawa¹ (1.Kwansei Gakuin Univ.)

Keywords:exosome, plasmon, fluorescence microscope

Exosome is one of the extracellular vesicles released from a cell. MicroRNAs and proteins are contained in exosomes, and therefore, studies of exosomes are known to be valuable for predicting diseases. In our laboratory, single exosomes have been detected by both of the epi-fluorescence and transmitted-light microscopy using a plasmonic chip, which is a wavelength-sized periodic structure substrate coated with a thin metal film. Plasmonic chip can provide the enhanced fluorescence by the grating-coupled surface plasmon resonance (GC-SPR). In this study, single exosome was detected using two types of membrane protein CD9 and CD81 with a plasmonic chip under the transmitted-light microscope.