[22a-P01-1~10] 1.3 Novel technologies and interdisciplinary engineering

[22a-P01-5] Development of label-free PCR by using plasmonic crystal

〇(M2)Futa Furukawa¹, Hirotaka Yamada¹, Daiki Kawasaki¹, Ryosuke Nishitsuji¹, Tatsuro Endo¹, Kenji Sueyoshi^1,2, Hideaki Hisamoto¹ (1.Osaka Pref. Univ., 2.JST PRESTO)

Keywords:Polymerase Chain Reaction, Plasmon, DNA analysis

The polymerase chain reaction (PCR) method, which is widely used in DNA analysis, has conventionally required complicated operations such as labeling and staining. In this study, simple and unlabeled PCR detection was performed by photodetection using a plasmonic crystal (PC). As an analysis method, a figure of merit (FOM) was adopted to confirm the overall change in each PCR cycle. As a result, a difference was confirmed between the positive control and the negative control, suggesting the possibility of unlabeled PCR detection by PC.