[24a-E104-1~8] 12.6 Nanobiotechnology

▲ [24a-E104-7] Investigation of Bacterial Adhesion on Various Surfaces using Quartz Crystal Microbalance with Energy Dissipation (QCM-D) Monitoring

〇(M1)Glenn Villena Latag¹, Taichi Nakamura¹, Evan Angelo Quimada Mondarte¹, Tomohiro Hayashi^1,2 (1.Tokyo TECH, 2.U. Tokyo)

Keywords:bacterial adhesion, quartz crystal microbalance, biointerface

Bacterial biofilm formation on biomedical devices poses serious risks to human life. The preliminary step in forming bacterial biofilms is the reversible attachment of the bacterial cells onto the surface. This is followed by bond maturation and secretion of polymeric substances, resulting in stable biofilms. This implies that understanding the reversible stage of the process is a crucial step to prevent biofilm formation. In this study, we analyze the adhesion processes of E. coli on various self-assembled monolayers (SAMs) with different terminal groups using QCM-D. We found that similar with proteins and platelets, a significant amount of bacterial cells adhere to hydrophobic and hydrophilic SAMs, while attaching weakly to protein resistant SAMs. Moreover, we observed positive frequency shifts on the protein-resistant SAMs at high harmonics, which suggest the manner at how bacterial cells clasp onto the surface using their appendages. We estimated the distance of the bacterial cell body from different surfaces. Elucidating how the bacterial cells adhere to different surface chemistries is a valuable step in designing biofilm prevention strategies.