16:15 〜 16:30
▲ [24p-D316-10] Push-pull system for single cell stimulation and real-time electrochemical detection of lactate response
キーワード：single cell, lactate, Microfludics
Metabolism of a single cell, even in the same organization, differs from each other by orders of magnitude.1 Single cell analysis can provide information on early diagnosis and localization of cancer.2 Conventional methods require complicated pre-treatment processes; thus they are difficult to authentically implement noninvasive detection of single cellular metabolites.
Herein, we present a liquid transfer based approach for in situ real time electrochemical detection which can detect metabolism of a single cell. In the fluid environment, cells ingest glucose and release lactate. Lactate is captured by nozzle system and finally decomposed by coupled multienzyme reaction system, the electrons generated in the reaction are captured by electrodes and converted into electrical signals. The push-pull nozzle system using layer-by-layer assembly technique shows high sensitivity, spatial-temporal resolution and enzyme specificity due to an increasing reaction area of accessible enzymes and a high transduction capacity of osmium polymer. This work provides a novel idea for detecting lactate secreted from single cells by non-invasive measurements.
Herein, we present a liquid transfer based approach for in situ real time electrochemical detection which can detect metabolism of a single cell. In the fluid environment, cells ingest glucose and release lactate. Lactate is captured by nozzle system and finally decomposed by coupled multienzyme reaction system, the electrons generated in the reaction are captured by electrodes and converted into electrical signals. The push-pull nozzle system using layer-by-layer assembly technique shows high sensitivity, spatial-temporal resolution and enzyme specificity due to an increasing reaction area of accessible enzymes and a high transduction capacity of osmium polymer. This work provides a novel idea for detecting lactate secreted from single cells by non-invasive measurements.