[24p-E104-1~16] CS.8 Code-sharing Session of 12.6 & 12.7

▲ [24p-E104-16] Development of automated paper-based femtogram sensing device for competitive ELISA of Aflatoxin B₁ using sub-microliter samples

〇(D)SUMAMAL CHARERNCHAI¹, Miyuki Chikae¹, Wanida Wonsawat², Daisuke Hirose¹, Yuzuru Takamura¹ (1.JAIST, 2.Suan Sunandha Rajabhat University)

Keywords:Paper-based device, Competitive ELISA, Small size molecule

In this work, we report a high-sensitivity (femtogram sensing) and low-volume sample (0.6 µL) paper-based device for automating a competitive enzyme-linked immunosorbent assay (ELISA) of a small-sized target, Aflatoxin B₁ (AFB₁), a potent carcinogen. Without requiring changes to the reaction components or structural design of the device, we demonstrated a novel approach to improve reaction and sensitivity by using the same zones of the antibody-conjugated enzyme (ALP-Ab) for sample loading before imersing the device into a running buffer. By using this approach, sample volume was reduced (> 100 times) while minimizing the possible loss of a target molecule by adsorption on the membrane, thus improving detection sensitivity.