[25p-E105-1~14] 8.4 Plasma life sciences

[25p-E105-8] Highly Efficient Gene Transfer in Cells by Combined Plasma and Pulsed Electric Field Treatment in Liquid

〇(DC)Ryosuke Honda¹, Shota Sasaki¹, Keisuke Takashima¹, Makoto Kanzaki², Takehiko Sato³, Toshiro Kaneko¹ (1.Grad. Sch. of Eng.,Tohoku Univ., 2.Grad. Sch. of Biomed. Eng.,Tohoku Univ., 3.Inst. of Fluid Sci.,Tohoku Univ.)

Keywords:Plasma in Liquid

We conducted experiments for the new molecular introduction method for cells in liquid phase by utilizing plasma in liquid. The treatment that combined plasma and pulsed electric field for cells enhanced GFP fluorescent expression in cultured cells. Therefore, it was considered that the plasmid DNA can be introduced into cells by the combined treatment and the treatment can be used for the new method of gene introduction.