[16p-A307-1~13] New horizon in interdisciplinary photonics pioneered by nano-photothermal conversion

[16p-A307-6] Development of Rapid and Sensitive Quantitative Analysis Method of DNA Based on Optical Condensation of Heterogeneous Probe Particles

〇Shuichi Toyouchi¹, Seiya Oomachi^1,2,3, Kota Hayashi^1,2,3, Yumiko Takagi¹, Mamoru Tamura^1,4, Shiho Tokonami^1,3, Takuya Iida^1,2 (1.Osaka Met. Univ. RILACS, 2.Grad. Sch. Sci.,Osaka Met. Univ., 3.Grad. Sch. Eng., Osaka Met. Univ., 4.Grad. Sch. Eng. Sci. Osaka Univ.)

Keywords:Light-induced acceralation, DNA detection, Hetero probe method

A principle of “light-induced acceleration” of molecular recognition using the synergistic effect of light-induced force and hydrodynamic interaction in a liquid containing probe particles with a molecular recognition mechanism has been proposed. We are utilizing the principle of light-induced acceleration for the development of a “Hetero-Probe Method” that enables rapid and highly sensitive DNA detection. In the presentation, we demonstrate the possibility of the hetero probe method for DNA quantitative detection by showing a calibration curve with high linearity, and further report the results of successful highly sensitive detection comparable to existing digital PCR by light irradiation for only 3 minutes.