[16p-PB03-1~20] 12.7 Biomedical Engineering and Biochips

[16p-PB03-10] Direct electron transfer biosensor strip with carbon nanotube and flavin adenine dinucleotide glucose dehydrogenase the second report

Taichi Tomai², Hisanori Iwasa⁴, Takeshi Tanaka³, Atsunori Hiratsuka³, Yohko Hoshino³, Katsumi Tsuji⁴, Takahide Kishimoto⁴, 〇Hitoshi Muguruma¹ (1.Juntendo Univ., 2.Shibaura Tech., 3.AIST, 4.Toyobo)

Keywords:biosensor strip, FAD glucose dehydrogenase, carbon nanotube

The low-potential operation (+0.05 V) of a direct electron transfer (DET) glucose biosensor strip with a small-volume electrochemical capillary chamber is demonstrated. We constructed a DET formation in which de-bundled carbon nanotubes were placed near the reaction center pocket of flavin adenine dinucleotide glucose dehydrogenase (FAD-GDH). The DET biosensor has the advantage of long-term stability, unlike an electron transfer mediator, which is naturally reduced to cause sensing malfunction in a mediated electron transfer biosensor, adopted in commercial devices. However, DET biosensors still have a high working potential owing to their overpotential. In this article, we have resolved this issue via a very short interval voltage application (+0.4 V) that reduced the onset potential, in other words, enhanced the current owing to an enzymatic reaction.