[17a-E102-1~11] 12.7 Biomedical Engineering and Biochips

[17a-E102-10] Investigation of protocol for liposome immobilization and sensing in microfluidic device-integrated biosensors

〇(M2)Yuya Takahashi¹, Kazuki Miyaoka¹, Kotaro Kamitani¹, Ryusuke Mitobe², Takumi Hasegawa², Carl Frederik Werner¹, Masayuki Sohgawa², Minoru Noda¹ (1.Kyoto Inst. Tech., 2.Niigata Univ.)

Keywords:microfluidics, micro-TAS, biosensor

In this study, we investigated a method of solution supply and signal measurement on a sensor substrate integrated with a microfluidic device. The microfluidic device was fabricated by forming a mold using a 3D printer and molding uncured PDMS. The ratio of main agent to hardener was changed from 10:1 to 20:1, and the PDMS was heated for 11 minutes to cure. After attaching it to the substrate it was heated again for about 20 minutes. Several types of solutions, such as ethanol, liposome-containing solution and phosphate-buffered saline (PBS) are used for immobilization and measurement and are needed to be passed through the microfluidic channel. For all steps, it was confirmed that no solution leaked out of the channel. The rate of change in strain resistance of the cantilever was measured in two cases where PBS was supplied without and with the addition of aSyn fibrils after the various solution supply and liposome immobilization operations described above. We confirmed that aSyn was detectable sufficiently. This suggests that the various solutions are successfully manipulated in the microfluidic device.