[18p-E102-1~13] 12.7 Biomedical Engineering and Biochips

△ [18p-E102-4] Fabrication of PVC membrane-based K⁺ image sensor using aluminum thin plate with nanoporous structure and investigation of spatial resolution

〇Keita Kuramochi¹, Hideo Doi¹, Tomoko Horio¹, Yong-Joon Choi¹, Kazuhiro Takahashi¹, Toshihiko Noda¹, Kazuaki Sawada¹ (1.Toyohashi Tech.)

Keywords:Potassium ion, Nanoporous alumina, Potentiometric sensor array

High-spatiotemporal imaging of extracellular potassium ion ([K⁺]_O) is important to understanding physiological and pathophysiological roles in brain. Toward high-resolution imaging of [K⁺]_O, we fabricated a 30 μm-pitch PVC membrane-based K⁺ image sensor using a thin aluminum plate with a nanoporous structure of 350 nm length and the imaging performance was evaluated. The fabricated image sensor responded to the K⁺ concentration dependence, and good sensitivity was obtained (45 mV/dec.). To verify the spatial resolution, we made a 4 mm square guide-based K⁺ gel likened to the brain tissue. Using the fabricated image sensor, the K⁺ response was measured by dropping K⁺ gel. It was found that the output image clearly changed in the aluminum thin plate-mounted sensor compared to that of the sensor without an aluminum thin plate, indicating that the spatial resolution for the K⁺ imaging may have been improved.