[18p-E102-1~13] 12.7 Biomedical Engineering and Biochips

△ [18p-E102-3] Fabrication of high-resolution multi-ion image sensor using rubber-based negative resist and extracellular ion imaging in the hippocampal slice

〇Jumpei Otsuka¹, Moe Kato¹, HIdeo Doi¹, Bijay Parajuli², Tomoko Horio¹, Eiji Shigetomi², Youichi Sinozaki², Yong-Joon Choi¹, Kazuhiro Takahashi¹, Toshiaki Hattori¹, Toshihiko Noda¹, Shuichi Koizumi², Kazuaki Sawada¹ (1.Toyohashi Univ. of Tech., 2.Yamanashi Univ.)

Keywords:Rubber-based resist, Multi-ion image sensor, Simultaneous visualization

In this study, we fabricated a 16 µm pitch high-resolution multi-ion image sensor with a K+-sensitive membrane patterned on a potentiometric array sensor using a rubber-based negative resist as a matrix material, and the detection performance was investigated. The fabricated image sensor exhibited good sensitivity with reasonable selectivity. Additionally, in the measurement experiment with drug stimulation using mouse brain tissue, we successfully visualized extracellular K+ and H+ distributions in the hippocampus, and simultaneous visualization was first demonstrated.